Journal article
Transforming growth factor-β1 requires NADPH oxidase 4 for angiogenesis in vitro and in vivo
HM Peshavariya, EC Chan, GS Liu, F Jiang, GJ Dusting
Journal of Cellular and Molecular Medicine | Published : 2014
DOI: 10.1111/jcmm.12263
Abstract
Angiogenesis, the formation of new blood vessels, is a key physiological event in organ development and tissue responses to hypoxia but is also involved in pathophysiologies such as tumour growth and retinopathies. Understanding the molecular mechanisms involved is important to design strategies for therapeutic intervention. One important regulator of angiogenesis is transforming growth factor-β1 (TGF-β1). In addition, reactive oxygen species (ROS) and the ROS-forming NADPH oxidase type 4 (Nox4) have been implicated as additional regulators such as during hypoxia. Here, we show that both processes are indeed mechanistically linked. TGF-β1-stimulated Nox4 expression and ROS formation in endot..
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Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
This study is supported by the National Health and Medical Research Council (NHMRC; 566911). HMP is Postdoctoral Fellow (PF 11M 6093) of the Heart Foundation Australia. GJD is a Principal Research Fellow of NHMRC (GNT0400303). FJ supported from Natural Science Foundation of China (81070164). The Centre for Eye Research Australia and O'Brien Institute acknowledge the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program. Authors are grateful to Prof. Harald HHW Schmidt and Dr. Kirstin Wingler for providing Nox4 knockout mice, and critical reading and suggestions for this manuscript. Adv-Nox4i and the anti-Nox4 antibody were kind gifts from Prof. John F Keaney Jr, University of Massachusetts Medical School and Prof. Ajay Shah, London, respectively.